Glutathione Peroxidase Activity in Human Erythrocytes and Plasma
نویسندگان
چکیده
منابع مشابه
Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes.
We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC 1.11.1.9), in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies for the assays gave CVs of 5.6% for a mean erythrocyte selenium concentration...
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In order to generate catalytic antibodies with glutathione peroxidase (GPx) activity, we prepared GSH-S-DNP butyl ester and GSH-S-DNP benzyl ester as the haptens. Two ScFvs that bound specifically to the haptens were selected from the human phage-displayed antibody library. The two ScFv genes were highly homologous, consisting of 786 bps and belonging to the same VH family-DP25. In the premise ...
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Human glutathione transferases (GSTs) from Alpha (A), Mu (M) and Theta (T) classes exhibited glutathione peroxidase activity towards phospholipid hydroperoxide. The specific activities are in the order: GST A1-1>GST T1-1>GST M1-1>GST A2-2>GST A4-4. Using a specific and sensitive HPLC method, specific activities towards the phospholipid hydroperoxide,1-palmitoyl-2-(13-hydroper oxy-cis-9, trans-1...
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Decreases in the glutathione peroxidase activity and in the selenium content were found in the plasma of pregnant women as compared with a control group. In the erythrocytes, both parameters remained unchanged. In both groups a fraction of about 0.90 of the glutathione peroxidase in the blood, was contained in the erythrocytes, whereas the selenium was nearly evenly distributed between plasma a...
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abstract background: the chemotherapeutic agents used for treatment of breast cancer are all shown to increase free oxygen radicals and generation of reactive oxygen species. the aim of this study was to evaluate effect of chemotherapy on plasma selenium (se) concentration and glutathione peroxidase (gpx) activity in breast cancer patients. materials and methods: seventeen women in stage ii and...
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ژورنال
عنوان ژورنال: Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene)
سال: 1983
ISSN: 0021-5082,1882-6482
DOI: 10.1265/jjh.38.581